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CRIMIC CR70650

Gene Dpck

CG1939

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	3R:7082182..7083251	84B2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTGTTCGCATTTACACTAACTGG	CGGAGCTGCTTGAGGAAC

Images

Construct Design Sequences

sgRNA sequence	GTTAAAATACTATAATTCGATGG
sgRNA2 sequence	GTGTCTGTGCGTGCCATACGAGG

 

left_200bp	right_200bp
TTGTGCTTTTATTATAAATACAACCATAACCGTTTTATTTTGTTTTTTAGAAACAGTTACGCAGAAGTGGCCCACAGTGCCCGCGAGTTTCAGTATTTTAAAAATAATTATTTAAACGCAAACGTGACCCTTGGTCACACTGTACCATTAGTACTTGAAAAATAAAACGAACAAAACGATAAAAAACATTCCTACCATCG	ACGAGGTCACTTTATGTGTTAATGCAAATGATACCAAACCAATTATACAATTTAAACGCTTGTTAGTGAAATATATTGCCGAAACGTGACAATCGCCTTTAATTAACAGTCTGAACAAACCTACATTGGTGTCGCGCTCACAAGGTAGAAGCCTAACAGACCATAAACGGAAGTGCCTTGAGATTGGATGCAGGGACAGA

 

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